Effect of culture media and skotoperiod on the germination of seeds and growth in vitro of Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae)
DOI:
https://doi.org/10.18387/polibotanica.56.8Keywords:
“La Candelaria”, Protocorms, Pre-germination, Oxidation, Coconut water, 6- benzylaminopurineAbstract
Guarianthe skinneri is subject to protection due to the loss of natural, wild populations, and the in vitro symbiotic germination of seeds is a viable alternative for the conservation and restoration of such populations. This laboratory process also facilitates the detailed study of the phases of germination, that, in the case of orchids, we now divide into two sections, pre-germination and germination, as a contribution towards the design of a strategy for the restoration of populations of this orchid. To that end, we evaluated four culture media, (Yasuda 10 % + 0.112 mg/L 6-bencilaminopurina (BAP), Yasuda 100 % + 1.125 mg/L BAP, modified Dalla Rosa & Laneri, Murashige & Skoog) and two skotoperiods (12 and 24 h), observing the phases of pre-germination and germination of the seeds in all treatments. The treatments with the culture media Dalla Rosa and Yasuda promoted the development and completion of the germination phases. The MS medium gave low percentages of development for phases 4 and 5, due to high percentages of oxidation in phases 2 and 3. The two skotoperiods did not affect the germination process.
References
Aguilar-Morales, M., & López-Escamilla, A. (2013). Germinación in vitro de Laelia speciosa (Kunth) Schltr., una herramienta para su conservación ex situ. Estudios Científicos En El Estado de Hidalgo y Zonas Aledañas. Vol. II. G. Pulido F. y S. Monks (Eds.). Zea Books, University of Nebraska. Lincoln, Nebraska, USA., 1(1), 18–24. http://digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1004&context=hidalgo
Andrade-Rodríguez, M., Vargas-Araujo, J., Gabriel Villegas-Torres, O., López-Martínez, V., Guillen-Sánchez Irán Alia-Tejacal, D., & Andrade Rodríguez, M. (2015). Germinación de semillas y crecimiento de plántulas de Cattleya (Brassolaeliocattleya) in vitro. Interciencia, 40(8), 549–553.
Arditti, J. (1982). Introduction, North American terrestrial orchids. Orchid Biology II. Reviews and Perspectives. Orchid Seed Germination and Seedling Culture -a Manual. P. 245-273.
Arditti, J., Karim, A., & Ghani, A. (2000). Numerical and physical properties of orchid seeds and their biological implications. New Phytol, 145, 367–421.
Ávila-Díaz, I., Oyama, K., Gómez-Alonso, C., & Salgado-Garciglia, R. (2009). In vitro propagation of the endangered orchid Laelia speciosa. Plant Cell, Tissue and Organ Culture, 99(3), 335–343. https://doi.org/10.1007/s11240-009-9609-8
Cabrera, T. (2006). Ficha técnica de Cattleya skinneri. Cuarenta y ocho especies de la flora de Chiapas incluidas en el PROY-NOM-059-ECOL-2000.
Cassells, A. C., & Curry, R. F. (2001). Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell, Tissue and Organ Culture, 64, 145-157. https://doi.org/10.1023/A:1010692104861
Castillo-Pérez, L. J., Martínez-Soto, D., Fortanelli-Martínez, J., & Carranza-Álvarez, C. (2021). Asymbiotic seed germination, in vitro seedling development, and symbiotic acclimatization of the Mexican threatened orchid Stanhopea tigrina. Plant Cell, Tissue and Organ Culture, 146(2), 249–257. https://doi.org/10.1007/s11240-021-02064-9
Chen, Y., Goodale, U. M., Fan, X.-L., & Gao, J.-Y. (2015). Asymbiotic seed germination and in vitro seedling development of Paphiopedilum spicerianum: An orchid with an extremely small population in China. Global Ecology and Conservation, 3, 367–378. https://doi.org/https://doi.org/10.1016/j.gecco.2015.01.002
Cruz-Higareda, J. B., Luna-Rosales, B. S., & Barba-Álvarez, A. (2015). A novel seed baiting technique for the epiphytic orchid Rhynchostele cervantesii, a means to acquire mycorrhizal fungi from protocorms. Lankesteriana, 15(1), 67–76. https://doi.org/10.15517/lank.v15i1.18525
Coello, C. Y., Miceli, C. L., Orantes, C., DenDooven L., & Gutiérrez, F. A. (2010). Optimización de reguladores de crecimiento para el cultivo in vitro de la orquídea Guarianthe skinneri (Bateman) Dressler & W.E. Higgins. Gayana Botánica 67 (1) 19-26.
Dalla Rosa, U., & Laneri, U. (1977). Modification of nutrient solutions for germination and growth in vitro of some cultivated orchids and for the vegetative propagation of Cymbidium cultivars. American Orchid Society Bulletin, 9, 813–820.
Damon, A. (2013). Conservation status and strategies for the preservation of orchids in the Soconusco Region of southeast Mexico. Lankesteriana, 13(1–2), 27–31. https://doi.org/10.15517/lank.v0i0.11530
Damon, A. (2017). Estrategia para el rescate, conservación y aprovechamiento sustentable de las orquídeas (Orchidaceae) en el sureste de México. Agroproductividad, 10(6), 25–30.
Damon, A., Aguilar-Guerrero, E., Rivera, L., & Nikolaeva, V. (2014). Germinación in vitro de semillas inmaduras de tres especies de orquídeas de la región del Soconusco, Chiapas, México. Revista Chapingo Serie Horticultura, 10(2), 195–203.
David, D., Jualang Azlan, G., & Ong Abdullah, J. (2008). Effect of NAA and BAP on protocorm proliferation of Borneo Scented Orchid. Asia-Pacific Journal of Molecular Biology and Biotechnology, 16(3), 221–224. https://www.researchgate.net/publication/259920952
Emeterio-Lara, A., García-Franco, J. G., Hernández-Apolinar, M., Mora-Herrera, M. E., Toledo-Hernández, V. H., Valencia-Díaz, S., & Flores-Palacios, A. (2018). Endogamy costs and reproductive biology of Laelia autumnalis, an endemic orchid of Mexico. Plant Ecology, 219(12), 1423–1434. https://doi.org/10.1007/s11258-018-0891-6
Fang, S., Chen, J., & Wei M. (2016). Protocorms and protocorm-like bodies are molecularly distinct from zygotic embryonic tissues in Phalaenopsis aphrodite. Plant Physiology 171(4), 2682-2700. https://doi.org/10.1104/pp.16.00841
García, J. A., Valerín, A. T., & Salazar, R. (1993). Utilización de tres medios orgánicos para la germinación in vitro de semillas de guaria morada, Cattleya skinneri (Bateman). Uniciencia, 79–83.
Hernández-Muñoz, S., Pedraza-Santos, M., López, P., de la Cruz-Torres, E., Martínez-Palacios, A., Fernández-Pavía, S., & Chávez-Bárcenas, A. (2017). Estimulación de la germinación y desarrollo in vitro de Laelia autumnalis con rayos gamma. Revista Fitotecnia Mexicana, 40(3), 217–283.
Hosomi, S. T., Santos, R. B., Custodio, C. C., Seaton, P. T., Marks, T. R., & Machado-Neto, N. B. (2011). Preconditioning Cattleya seeds to improve the efficacy of the tetrazolium test for viability. Seed Science and Technology, 39(1), 178–189. https://doi.org/10.15258/sst.2011.39.1.15
Johnson, T. R., & Kane, M. E. (2007). Asymbiotic germination of ornamental Vanda: In vitro germination and development of three hybrids. Plant Cell, Tissue and Organ Culture, 91(3), 251–261. https://doi.org/10.1007/s11240-007-9291-7
Kauth, P., Dutra, D., Johnson, T., Stewart, S., Kane, M., & Vendrame, W. (2008). Techniques and applications of in vitro orchid seed germination. Floriculture, Ornamental and Plant Biotechnology, 375–391. https://www.researchgate.net/publication/257385365
Koene, F. M., Amano, É., de Camargo Smidt, E., & Ribas, L. L. F. (2020). Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae). PLoS ONE, 15(12), 1–17. https://doi.org/10.1371/journal.pone.0243297
López, V. A., Mejía, M. J., & Sosa-Moss, C. (1994). Cultivo de tejidos de orquídeas: Cattleya, Encyclia, Oncidium y Stanhopea. Revista Chapingo Serie Horticultura, 1, 58–62.
López-Chávez, M. Y., Guillén-Navarro, K., Bertolini, V., Encarnación, S., Hernández-Ortiz, M., Sánchez-Moreno, I., & Damon, A. (2016). Proteomic and morphometric study of the in vitro interaction between Oncidium sphacelatum Lindl. (Orchidaceae) and Thanatephorus sp. RG26 (Ceratobasidiaceae). Mycorrhiza, 26(5), 353–365. https://doi.org/10.1007/s00572-015-0676-x
Ma, Z., Ge, L., Lee, A. S. Y., Yong, J. W. H., Tan, S. N., & Ong, E. S. (2008). Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry after solid-phase extraction. Analytica Chimica Acta, 610(2), 274–281. https://doi.org/https://doi.org/10.1016/j.aca.2008.01.045
Maldonado, G. P., Yarzábal, L. A., Cevallos-Cevallos, J. M., Chica, E. J., & Peña, D. F. (2020). Root endophytic fungi promote in vitro seed germination in Pleurothallis coriacardia (Orchidaceae). Lankesteriana, 20(1), 107–122. https://doi.org/10.15517/LANK.V20I1.41472
Mercado, S. A. S., & Contreras, N. A. V. (2017). Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae). Acta Agronomica, 66(4), 544–548. https://doi.org/10.15446/acag.v66n4.63597
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Plant Physiology, 15(4), 31. https://doi.org/10.1104/pp.113.231753
Ortiz, C. S., González, C. G., López, G. P., & Donjuan, L. I. (2013). Efecto de sustratos, soluciones nutritivas y tamaño de vitroplanta en aclimatación de Candelaria (Guarianthe skinneri). Memorias de Congreso Nacional y Primer Congreso Internacional de La Sociedad Mexicana de Ciencias Hortícolas A.C.
Quiroz, K., Saavedra, J., Vogel, H., Verdugo, G., Caligari, P. D. S., & García-Gonzáles, R. (2017). In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae). Applications in Plant Sciences, 5(8), 1600142. https://doi.org/10.3732/apps.1600142
Rasmussen, H. N., Dixon, K. W., Jersáková, J., & Těšitelová, T. (2015). Germination and seedling establishment in orchids: A complex of requirements. Annals of Botany, 116(3), 391–402. https://doi.org/10.1093/aob/mcv087
Restrepo, O. C., Gómez, V. F., Gil, C. A., Torres, B. J., & Urrea, T. A. (2018). In vitro propagation of avocado (Persea americana Mill.) cv. Hass through morphogenesis. Acta Agronómica, 67(1) 160-167. DOI: https://doi.org/10.1S446/acag.v67n1.61474
Salazar, S. A., & Osorio, Y. M. (2022). Implementation of organic components to the culture medium to improve the in vitro propagation of Cattleya warscewiczii and Cattleya gaskelliana. South African Journal of Botany, 148, 352–359. https://doi.org/10.1016/j.sajb.2022.05.002
Salazar-Mercado, S. (2012). Germinación asimbiótica de semillas y desarrollo in vitro de plántulas de Cattleya mendelii Dombrain (Orchidaceae). Acta Agronómica, 61(1), 69–78.
Solano-Gómez, R., Damon, A., Cruz-Lustre, G., Jiménez-Bautista, L., Avendaño-Vázquez, S., Bertolini, V., Rivera-García, R., & Cruz-García, G. (2016). Diversity and distribution of the orchids of the Tacaná-Boquerón region, Chiapas, Mexico. Botanical Sciences, 94(3), 625–656. https://doi.org/10.17129/botsci.589
Stewart, S. L., & Kane, M. E. (2006). Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell, Tissue and Organ Culture, 86(2), 147–158. https://doi.org/10.1007/s11240-006-9098-y
Teixeira da Silva, J. A., Musharof, H.M., Sharma, M., Dobránski, J., Cardoso, J. C., & Zeng, S. 2017. Acclimatization of in vitro derived Dendrobium. Horticultural Plant Journal 3(3), 110–124. https://doi.org/10.1016/j.hpj.2017.07.009.
Tomas, P., & Ravindra, B. (1997). Shoot tip culture in mango: Influence of medium, genotype, explant factors, season and decontamination treatments on phenolic exudation, explant survival and axenic culture establishment. Journal of Horticultural science, 72(5), 713-722. https://doi.org/10.1080/14620316.1997.11515563.
Torres-González, M. J., Aguirre-Medina, J. F., & Iracheta-Donjuan, L. (2011). Germinación de semilla y obtención de plántulas de Vanilla planifolia Andrews en condiciones in vitro. AGROProductividad, 4(2), 3–8.
Utami, E. S. W., & Hariyanto, S. (2019). In vitro seed germination and seedling development of a rare indonesian native orchid Phalaenopsis amboinensis J.J.Sm. Scientifica. https://doi.org/10.1155/2019/8105138
Utami, E. S. W., & Hariyanto, S. (2020). Organic compounds: contents and their role in improving seed germination and protocorm development in orchids. International Journal of Agronomy. https://doi.org/10.1155/2020/2795108
Valadares, R. B. S., Perotto, S., Santos, E. C., & Lambais, M. R. (2014). Proteome changes in Oncidium sphacelatum (Orchidaceae) at different trophic stages of symbiotic germination. Mycorrhiza, 24(5), 349–360. https://doi.org/10.1007/s00572-013-0547-2
Vasudevan, R., & van Staden, J. (2010). In vitro asymbiotic seed germination and seedling growth of Ansellia africana Lindl. Scientia Horticulturae, 123(4), 496–504. https://doi.org/10.1016/j.scienta.2009.11.010
Yasuda, T., Fujii, Y., & Yamaguchi, T. (1985). Embryogenic callus induction from Coffea arabica leaf explants by benzyladenine. Plant and Cell Physiology, 26(3), 595–597. https://doi.org/10.1093/oxfordjournals.pcp.a076946
Yeung, E. C. (2017). A perspective on orchid seed and protocorm development. Botanical Studies, 58(1), 1–14. https://doi.org/10.1186/s40529-017-0188-4
Zeng, S., Wu, K., Teixeira da Silva, J. A., Zhang, J., Chen, Z., Xia, N., & Duan, J. (2016). Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid. Scientia Horticulturae, 138, 198–209. https://doi.org/https://doi.org/10.1016/j.scienta.2012.02.026
Zhang, Y. Y., Wu, K. L., Zhang, J. X., Deng, R. F., Duan, J., Teixeira Da Silva, J. A., Huang, W. C., & Zeng, S. J. (2015). Embryo development in association with asymbiotic seed germination in vitro of Paphiopedilum armeniacum S. C. Chen et F. Y. Liu. Scientific Reports, 5. https://doi.org/10.1038/srep16356
Andrade-Rodríguez, M., Vargas-Araujo, J., Gabriel Villegas-Torres, O., López-Martínez, V., Guillen-Sánchez Irán Alia-Tejacal, D., & Andrade Rodríguez, M. (2015). Germinación de semillas y crecimiento de plántulas de Cattleya (Brassolaeliocattleya) in vitro. Interciencia, 40(8), 549–553.
Arditti, J. (1982). Introduction, North American terrestrial orchids. Orchid Biology II. Reviews and Perspectives. Orchid Seed Germination and Seedling Culture -a Manual. P. 245-273.
Arditti, J., Karim, A., & Ghani, A. (2000). Numerical and physical properties of orchid seeds and their biological implications. New Phytol, 145, 367–421.
Ávila-Díaz, I., Oyama, K., Gómez-Alonso, C., & Salgado-Garciglia, R. (2009). In vitro propagation of the endangered orchid Laelia speciosa. Plant Cell, Tissue and Organ Culture, 99(3), 335–343. https://doi.org/10.1007/s11240-009-9609-8
Cabrera, T. (2006). Ficha técnica de Cattleya skinneri. Cuarenta y ocho especies de la flora de Chiapas incluidas en el PROY-NOM-059-ECOL-2000.
Cassells, A. C., & Curry, R. F. (2001). Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell, Tissue and Organ Culture, 64, 145-157. https://doi.org/10.1023/A:1010692104861
Castillo-Pérez, L. J., Martínez-Soto, D., Fortanelli-Martínez, J., & Carranza-Álvarez, C. (2021). Asymbiotic seed germination, in vitro seedling development, and symbiotic acclimatization of the Mexican threatened orchid Stanhopea tigrina. Plant Cell, Tissue and Organ Culture, 146(2), 249–257. https://doi.org/10.1007/s11240-021-02064-9
Chen, Y., Goodale, U. M., Fan, X.-L., & Gao, J.-Y. (2015). Asymbiotic seed germination and in vitro seedling development of Paphiopedilum spicerianum: An orchid with an extremely small population in China. Global Ecology and Conservation, 3, 367–378. https://doi.org/https://doi.org/10.1016/j.gecco.2015.01.002
Cruz-Higareda, J. B., Luna-Rosales, B. S., & Barba-Álvarez, A. (2015). A novel seed baiting technique for the epiphytic orchid Rhynchostele cervantesii, a means to acquire mycorrhizal fungi from protocorms. Lankesteriana, 15(1), 67–76. https://doi.org/10.15517/lank.v15i1.18525
Coello, C. Y., Miceli, C. L., Orantes, C., DenDooven L., & Gutiérrez, F. A. (2010). Optimización de reguladores de crecimiento para el cultivo in vitro de la orquídea Guarianthe skinneri (Bateman) Dressler & W.E. Higgins. Gayana Botánica 67 (1) 19-26.
Dalla Rosa, U., & Laneri, U. (1977). Modification of nutrient solutions for germination and growth in vitro of some cultivated orchids and for the vegetative propagation of Cymbidium cultivars. American Orchid Society Bulletin, 9, 813–820.
Damon, A. (2013). Conservation status and strategies for the preservation of orchids in the Soconusco Region of southeast Mexico. Lankesteriana, 13(1–2), 27–31. https://doi.org/10.15517/lank.v0i0.11530
Damon, A. (2017). Estrategia para el rescate, conservación y aprovechamiento sustentable de las orquídeas (Orchidaceae) en el sureste de México. Agroproductividad, 10(6), 25–30.
Damon, A., Aguilar-Guerrero, E., Rivera, L., & Nikolaeva, V. (2014). Germinación in vitro de semillas inmaduras de tres especies de orquídeas de la región del Soconusco, Chiapas, México. Revista Chapingo Serie Horticultura, 10(2), 195–203.
David, D., Jualang Azlan, G., & Ong Abdullah, J. (2008). Effect of NAA and BAP on protocorm proliferation of Borneo Scented Orchid. Asia-Pacific Journal of Molecular Biology and Biotechnology, 16(3), 221–224. https://www.researchgate.net/publication/259920952
Emeterio-Lara, A., García-Franco, J. G., Hernández-Apolinar, M., Mora-Herrera, M. E., Toledo-Hernández, V. H., Valencia-Díaz, S., & Flores-Palacios, A. (2018). Endogamy costs and reproductive biology of Laelia autumnalis, an endemic orchid of Mexico. Plant Ecology, 219(12), 1423–1434. https://doi.org/10.1007/s11258-018-0891-6
Fang, S., Chen, J., & Wei M. (2016). Protocorms and protocorm-like bodies are molecularly distinct from zygotic embryonic tissues in Phalaenopsis aphrodite. Plant Physiology 171(4), 2682-2700. https://doi.org/10.1104/pp.16.00841
García, J. A., Valerín, A. T., & Salazar, R. (1993). Utilización de tres medios orgánicos para la germinación in vitro de semillas de guaria morada, Cattleya skinneri (Bateman). Uniciencia, 79–83.
Hernández-Muñoz, S., Pedraza-Santos, M., López, P., de la Cruz-Torres, E., Martínez-Palacios, A., Fernández-Pavía, S., & Chávez-Bárcenas, A. (2017). Estimulación de la germinación y desarrollo in vitro de Laelia autumnalis con rayos gamma. Revista Fitotecnia Mexicana, 40(3), 217–283.
Hosomi, S. T., Santos, R. B., Custodio, C. C., Seaton, P. T., Marks, T. R., & Machado-Neto, N. B. (2011). Preconditioning Cattleya seeds to improve the efficacy of the tetrazolium test for viability. Seed Science and Technology, 39(1), 178–189. https://doi.org/10.15258/sst.2011.39.1.15
Johnson, T. R., & Kane, M. E. (2007). Asymbiotic germination of ornamental Vanda: In vitro germination and development of three hybrids. Plant Cell, Tissue and Organ Culture, 91(3), 251–261. https://doi.org/10.1007/s11240-007-9291-7
Kauth, P., Dutra, D., Johnson, T., Stewart, S., Kane, M., & Vendrame, W. (2008). Techniques and applications of in vitro orchid seed germination. Floriculture, Ornamental and Plant Biotechnology, 375–391. https://www.researchgate.net/publication/257385365
Koene, F. M., Amano, É., de Camargo Smidt, E., & Ribas, L. L. F. (2020). Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae). PLoS ONE, 15(12), 1–17. https://doi.org/10.1371/journal.pone.0243297
López, V. A., Mejía, M. J., & Sosa-Moss, C. (1994). Cultivo de tejidos de orquídeas: Cattleya, Encyclia, Oncidium y Stanhopea. Revista Chapingo Serie Horticultura, 1, 58–62.
López-Chávez, M. Y., Guillén-Navarro, K., Bertolini, V., Encarnación, S., Hernández-Ortiz, M., Sánchez-Moreno, I., & Damon, A. (2016). Proteomic and morphometric study of the in vitro interaction between Oncidium sphacelatum Lindl. (Orchidaceae) and Thanatephorus sp. RG26 (Ceratobasidiaceae). Mycorrhiza, 26(5), 353–365. https://doi.org/10.1007/s00572-015-0676-x
Ma, Z., Ge, L., Lee, A. S. Y., Yong, J. W. H., Tan, S. N., & Ong, E. S. (2008). Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry after solid-phase extraction. Analytica Chimica Acta, 610(2), 274–281. https://doi.org/https://doi.org/10.1016/j.aca.2008.01.045
Maldonado, G. P., Yarzábal, L. A., Cevallos-Cevallos, J. M., Chica, E. J., & Peña, D. F. (2020). Root endophytic fungi promote in vitro seed germination in Pleurothallis coriacardia (Orchidaceae). Lankesteriana, 20(1), 107–122. https://doi.org/10.15517/LANK.V20I1.41472
Mercado, S. A. S., & Contreras, N. A. V. (2017). Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae). Acta Agronomica, 66(4), 544–548. https://doi.org/10.15446/acag.v66n4.63597
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Plant Physiology, 15(4), 31. https://doi.org/10.1104/pp.113.231753
Ortiz, C. S., González, C. G., López, G. P., & Donjuan, L. I. (2013). Efecto de sustratos, soluciones nutritivas y tamaño de vitroplanta en aclimatación de Candelaria (Guarianthe skinneri). Memorias de Congreso Nacional y Primer Congreso Internacional de La Sociedad Mexicana de Ciencias Hortícolas A.C.
Quiroz, K., Saavedra, J., Vogel, H., Verdugo, G., Caligari, P. D. S., & García-Gonzáles, R. (2017). In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae). Applications in Plant Sciences, 5(8), 1600142. https://doi.org/10.3732/apps.1600142
Rasmussen, H. N., Dixon, K. W., Jersáková, J., & Těšitelová, T. (2015). Germination and seedling establishment in orchids: A complex of requirements. Annals of Botany, 116(3), 391–402. https://doi.org/10.1093/aob/mcv087
Restrepo, O. C., Gómez, V. F., Gil, C. A., Torres, B. J., & Urrea, T. A. (2018). In vitro propagation of avocado (Persea americana Mill.) cv. Hass through morphogenesis. Acta Agronómica, 67(1) 160-167. DOI: https://doi.org/10.1S446/acag.v67n1.61474
Salazar, S. A., & Osorio, Y. M. (2022). Implementation of organic components to the culture medium to improve the in vitro propagation of Cattleya warscewiczii and Cattleya gaskelliana. South African Journal of Botany, 148, 352–359. https://doi.org/10.1016/j.sajb.2022.05.002
Salazar-Mercado, S. (2012). Germinación asimbiótica de semillas y desarrollo in vitro de plántulas de Cattleya mendelii Dombrain (Orchidaceae). Acta Agronómica, 61(1), 69–78.
Solano-Gómez, R., Damon, A., Cruz-Lustre, G., Jiménez-Bautista, L., Avendaño-Vázquez, S., Bertolini, V., Rivera-García, R., & Cruz-García, G. (2016). Diversity and distribution of the orchids of the Tacaná-Boquerón region, Chiapas, Mexico. Botanical Sciences, 94(3), 625–656. https://doi.org/10.17129/botsci.589
Stewart, S. L., & Kane, M. E. (2006). Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell, Tissue and Organ Culture, 86(2), 147–158. https://doi.org/10.1007/s11240-006-9098-y
Teixeira da Silva, J. A., Musharof, H.M., Sharma, M., Dobránski, J., Cardoso, J. C., & Zeng, S. 2017. Acclimatization of in vitro derived Dendrobium. Horticultural Plant Journal 3(3), 110–124. https://doi.org/10.1016/j.hpj.2017.07.009.
Tomas, P., & Ravindra, B. (1997). Shoot tip culture in mango: Influence of medium, genotype, explant factors, season and decontamination treatments on phenolic exudation, explant survival and axenic culture establishment. Journal of Horticultural science, 72(5), 713-722. https://doi.org/10.1080/14620316.1997.11515563.
Torres-González, M. J., Aguirre-Medina, J. F., & Iracheta-Donjuan, L. (2011). Germinación de semilla y obtención de plántulas de Vanilla planifolia Andrews en condiciones in vitro. AGROProductividad, 4(2), 3–8.
Utami, E. S. W., & Hariyanto, S. (2019). In vitro seed germination and seedling development of a rare indonesian native orchid Phalaenopsis amboinensis J.J.Sm. Scientifica. https://doi.org/10.1155/2019/8105138
Utami, E. S. W., & Hariyanto, S. (2020). Organic compounds: contents and their role in improving seed germination and protocorm development in orchids. International Journal of Agronomy. https://doi.org/10.1155/2020/2795108
Valadares, R. B. S., Perotto, S., Santos, E. C., & Lambais, M. R. (2014). Proteome changes in Oncidium sphacelatum (Orchidaceae) at different trophic stages of symbiotic germination. Mycorrhiza, 24(5), 349–360. https://doi.org/10.1007/s00572-013-0547-2
Vasudevan, R., & van Staden, J. (2010). In vitro asymbiotic seed germination and seedling growth of Ansellia africana Lindl. Scientia Horticulturae, 123(4), 496–504. https://doi.org/10.1016/j.scienta.2009.11.010
Yasuda, T., Fujii, Y., & Yamaguchi, T. (1985). Embryogenic callus induction from Coffea arabica leaf explants by benzyladenine. Plant and Cell Physiology, 26(3), 595–597. https://doi.org/10.1093/oxfordjournals.pcp.a076946
Yeung, E. C. (2017). A perspective on orchid seed and protocorm development. Botanical Studies, 58(1), 1–14. https://doi.org/10.1186/s40529-017-0188-4
Zeng, S., Wu, K., Teixeira da Silva, J. A., Zhang, J., Chen, Z., Xia, N., & Duan, J. (2016). Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid. Scientia Horticulturae, 138, 198–209. https://doi.org/https://doi.org/10.1016/j.scienta.2012.02.026
Zhang, Y. Y., Wu, K. L., Zhang, J. X., Deng, R. F., Duan, J., Teixeira Da Silva, J. A., Huang, W. C., & Zeng, S. J. (2015). Embryo development in association with asymbiotic seed germination in vitro of Paphiopedilum armeniacum S. C. Chen et F. Y. Liu. Scientific Reports, 5. https://doi.org/10.1038/srep16356
Downloads
Published
2023-07-04
Issue
Section
Articles
License
Polibotánica by Departamento de Botánica de la Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional se distribuye bajo una Licencia Creative Commons Atribución-NoComercial-CompartirIgual 4.0 Internacional.
How to Cite
Effect of culture media and skotoperiod on the germination of seeds and growth in vitro of Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae). (2023). POLIBOTANICA, 56. https://doi.org/10.18387/polibotanica.56.8
