Micropropagación del lirio amazónico (Eucharis grandiflora Planch. & Linden) mediante organogénesis directa.
MULTIPLICACIÓN in vitro DEL LIRIO AMAZÓNICO
Palabras clave:
Amaryllidaceae, biotecnología, propagación, bulbo
Resumen
El lirio amazónico (Eucharis grandiflora) es una especie cuyas características ofrecen un enorme potencial de uso ornamental, por lo que se buscó contar con un protocolo in vitro eficiente tanto para la obtención y multiplicación de plántulas. Se emplearon las sales inorgánicas del medio básico de Murashige & Skoog (1962) al 100%, suplementadas con 0.40 mg·L-1 de tiamina, 100 mg·L-1 de inositol, 80 mg·L-1 de L-cisteína, y 30 g·L-1 de sacarosa. En la multiplicación in vitro de plántulas, se evaluaron diferentes niveles de BAP en dosis de 0.0, 0.1, 0.3, 1.0 y 3.0 mg·L-1. Para el enraizamiento de plántulas, se emplearon diferentes dosis de AIA, AIB y ANA, en intervalos de 0.0, 0.3 y 1.0 mg·L-1. Para promover un mejor desarrollo de los brotes, las mejores respuestas se observaron en medios sin la presencia de reguladores del crecimiento y evaluando el contenido de sacarosa en concentraciones de 30 a 70 g·L-1. Para la fase de aclimatización de plántulas obtenidas in vitro, fueron colocadas en contenedores de 1 L con turba de musgo. En lo resultados obtenidos en la fase de establecimiento de explantes, se obtuvieron porcentajes del 30% de contaminación. Durante la multiplicación de plántulas, el uso de BAP con una dosis de 3.0 mg·L-1 produjo un máximo de 3.8 brotes por explante. El mejor desarrollo de los bulbillos se obtuvo con 50 g·L-1 de sacarosa. Durante el enraizamiento el AIA y AIB no produjeron significativamente más raíces que el testigo, aunque se observó una mayor longitud de éstas. Además, la adición del ANA promovió la formación de un mayor número de raíces, y un menor vigor. Durante la aclimatización en invernadero, se logró un 100% de sobrevivencia de plántulas producidas a los 50 días.
Citas
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Bach, A., & Sochacki, D. (2012). Propagation of ornamental geophytes: Physiology and management. In R. Kamenetsky & H. Okubo (Eds.), Ornamental geophytes: From basic science to sustainable production (pp. 261–286). https://doi.org/https://doi.org/10.1201/b12881
Baskaran, P., & Van Staden, J. (2013). Rapid in vitro micropropagation of Agapanthus praecox. South African Journal of Botany, 86, 46–50. https://doi.org/10.1016/j.sajb.2013.01.008
Beyl, C. A., & Trigiano, R. N. (2015). Plant propagation concepts and laboratory exercises (2nd ed.). https://doi.org/10.1201/b17340
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Çığ, A., & Başdoğan, G. (2015). In vitro propagation techniques for some geophyte ornamental plants with high economic value. International Journal of Secondary Metabolite, 2(1), 27–49.
Davies, F. T., Geneve, R. L., Wilson, S. B., Hartmann, H. T., & Kester, D. E. (2018). Hartmann & Kester’s plant propagation : principles and practices (9th ed.). Pearson.
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de Klerk, G. J. (2012). Micropropagation of bulbous crops: technology and present state. Floriculture and Ornamental Biotechnology, 6(1), 1–8.
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Evangelista-Lozano, S., Estrada-García, M., Escobar-Arellano, S. L., Arenas-Ocampo, M. L., Camacho-Díaz, B. H., & Jiménez-Aparicio, A. R. (2015). Eucharis Grandiflora Planch. and Linden development fertirrigated with different osmotic potentials. Journal of Plant Nutrition, 38(4), 551–567. https://doi.org/10.1080/01904167.2014.934467
Fennell, C. W., Crouch, N. R., & van Staden, J. (2001). Micropropagation of the river lily, Crinum variabile (Amaryllidaceae). South African Journal of Botany, 67(1), 74–77. https://doi.org/10.1016/S0254-6299(15)31096-6
George, E. F., Hall, M. A., & De Klerk, G. J. (2008). Plant propagation by tissue culture (3rd ed., Vol. 1). https://doi.org/10.1007/978-1-4020-5005-3
Grootaarts, H., Schel, J. H. N., & Pierik, R. L. M. (1981). The origin of bulblets formed on excised twin scales of Nerine bowdenii W. Watts. Plant Cell, Tissue and Organ Culture, 1(1), 39–46. https://doi.org/10.1007/BF02318902
Hasegawa, A., Takigawa, Y., & Tokuzumi, A. (1995). Propagation of Eucharis grandiflora Planch. by tissue culture. Technical Bulletin of Faculty of Agriculture-Kagawa University (Japan), 47(1), 79–86.
Hong, J., & Lee, A. K. (2012). Micropropagation of Cyrtanthus ’Orange Gem’×C. eucallus hybrid. Scientia Horticulturae, 142, 174–179. https://doi.org/10.1016/j.scienta.2012.05.016
Meerow, A. W. (1989). Systematics of the Amazon Lilies, Eucharis and Caliphruria (Amaryllidaceae). Annals of the Missouri Botanical Garden, 76(1), 220. https://doi.org/10.2307/2399347
Meerow, A. W., Jost, L., & Oleas, N. (2015). Two new species of endemic ecuadorean Amaryllidaceae (Asparagales, Amaryllidaceae, Amarylloideae, Eucharideae). PhytoKeys, 48(1), 1–9. https://doi.org/10.3897/phytokeys.48.4399
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Ozel, C. A., Khawar, K. M., & Unal, F. (2015). Factors affecting efficient in vitro micropropagation of Muscari muscarimi Medikus using twin bulb scale. Saudi Journal of Biological Sciences, 22(2), 132–138. https://doi.org/10.1016/j.sjbs.2014.09.007
Paredes, K., Delaveau, C., Carrasco, P., Baeza, C., Mora, F., & Uribe, M. E. (2014). In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile. Ciencia e Investigacion Agraria, 41(2), 207–214. https://doi.org/10.4067/S0718-16202014000200007
Pierik, R. L. M., Sprenkels, P. A., & van Bragt, J. (1983). Rapid vegetative propagation of Eucharis grandiflora in vitro. Acta Horticulturae, (147), 179–186. https://doi.org/10.17660/actahortic.1983.147.25
Rodrigues-Martins, J. P., Pasqual, M., Martins, A. D., & Ribeira, S. F. (2015). Effects of salts and sucrose concentrations on in vitro propagation of Billbergia zebrina (Herbert) Lindley (Bromeliaceae). Australian Journal of Crop Science, 9(1), 85–91.
Santos, A., Fidalgo, F., Santos, I., & Salema, R. (2002). In vitro bulb formation of Narcissus asturiensis, a threatened species of the Amaryllidaceae. Journal of Horticultural Science and Biotechnology, 77(2), 149–152. https://doi.org/10.1080/14620316.2002.11511471
Siwach, P., Grover, K., & Gill, A. R. (2011). The influence of plant growth regulators, explant nature and sucrose concentration on in vitro callus growth of Thevetia peruviana Schum. Asian Journal of Biotechnology, 3(3), 280–292. https://doi.org/10.3923/ajbkr.2011.280.292
Smith, R. H., Burrows, J., & Kurten, K. (1999). Challenges associated with micropropagation of Zephyranthes and Hippesatrum sp. (Amaryllidaceae). In Vitro Cellular and Developmental Biology - Plant, 35(4), 281–282. https://doi.org/10.1007/s11627-999-0032-y
Sultana, J., Sultana, N., Siddique, M. N. A., Islam, A. K. M. A., Hossain, M. M., & Hossain, T. (2010). In vitro bulb production in Hippeastrum (Hippeastrum hybridum). Journal of Central European Agriculture, 11(4), 469–474. https://doi.org/https://doi.org/10.5513/JCEA01/11.4.867
Tipirtamaz, R. (2003). Rooting and acclimatization of in vitro micropropagated snowdrop (Galanthus ikariae Baker.) bulblets. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 16(2), 121–126.
Trigiano, R. N., & Gray, D. J. (2004). Plant development and biotechnology. https://doi.org/10.1201/9780203506561
Van Dijk, H., & Kurpershoek, M. (2003). La enciclopedia de las plantas bulbosas. Editorial LIBSA.
Vargas, T. E., Oropeza, M., & de García, E. (2006). Propagación in vitro de Hippeastrum sp. Agronomía Tropical, 56(4), 621–626.
Yusti-Muñoz, A. P., & Velandia-Perilla, J. H. (2013). First record of the amazon lily Eucharis sanderi (Amaryllidaceae) for Isla Gorgona, Colombia. Boletín Científico. Centro de Museos. Museo de Historia Natural, 17(1), 46–49.
Ziv, M., & Lilien-Kipnis, H. (2000). Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant Cell Reports, 19(9), 845–850. https://doi.org/10.1007/s002990000204
Al-Khateeb, A. A. (2008). Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99(14), 6550–6555. https://doi.org/10.1016/j.biortech.2007.11.070
Anis, M., & Ahmad, N. (2016). Plant tissue culture: Propagation, conservation and crop improvement. https://doi.org/10.1007/978-981-10-1917-3
Bach, A., & Sochacki, D. (2012). Propagation of ornamental geophytes: Physiology and management. In R. Kamenetsky & H. Okubo (Eds.), Ornamental geophytes: From basic science to sustainable production (pp. 261–286). https://doi.org/https://doi.org/10.1201/b12881
Baskaran, P., & Van Staden, J. (2013). Rapid in vitro micropropagation of Agapanthus praecox. South African Journal of Botany, 86, 46–50. https://doi.org/10.1016/j.sajb.2013.01.008
Beyl, C. A., & Trigiano, R. N. (2015). Plant propagation concepts and laboratory exercises (2nd ed.). https://doi.org/10.1201/b17340
Cardoso, J. C., Sheng Gerald, L. T., & Teixeira da Silva, J. A. (2018). Micropropagation in the twenty-first century. In V. M. Loyola-Vargas & N. Ochoa-Alejo (Eds.), Methods in Molecular Biology (Vol. 1815, pp. 17–46). https://doi.org/10.1007/978-1-4939-8594-4_2
Cázarez-Prado, M., Andrade-Rodríguez, M., Villegas-Monter, Á., Alia-Tejacal, I., Villegas-Torres, Ó. G., & López-Martínez, V. (2010). In vitro propagation of Sprekelia formosissina Herbert., a wild plant with ornamental potential. Revista Fitotecnia Mexicana, 33(3), 197–203.
Çığ, A., & Başdoğan, G. (2015). In vitro propagation techniques for some geophyte ornamental plants with high economic value. International Journal of Secondary Metabolite, 2(1), 27–49.
Davies, F. T., Geneve, R. L., Wilson, S. B., Hartmann, H. T., & Kester, D. E. (2018). Hartmann & Kester’s plant propagation : principles and practices (9th ed.). Pearson.
De Bruyn, M. H., Ferreira, D. I., Slabbert, M. M., & Pretorius, J. (1992). In vitro propagation of Amaryllis belladonna. Plant Cell, Tissue and Organ Culture, 31(3), 179–184. https://doi.org/10.1007/BF00036221
de Klerk, G. J. (2012). Micropropagation of bulbous crops: technology and present state. Floriculture and Ornamental Biotechnology, 6(1), 1–8.
Di Rienzo, J. A., Casanoves, F., Balzarini, M. G., González, L., Tablada, M., & Robledo, C. W. (2016). InfoStat versión 2016. Retrieved from http://www.infostat.com.ar
Evangelista-Lozano, S., Estrada-García, M., Escobar-Arellano, S. L., Arenas-Ocampo, M. L., Camacho-Díaz, B. H., & Jiménez-Aparicio, A. R. (2015). Eucharis Grandiflora Planch. and Linden development fertirrigated with different osmotic potentials. Journal of Plant Nutrition, 38(4), 551–567. https://doi.org/10.1080/01904167.2014.934467
Fennell, C. W., Crouch, N. R., & van Staden, J. (2001). Micropropagation of the river lily, Crinum variabile (Amaryllidaceae). South African Journal of Botany, 67(1), 74–77. https://doi.org/10.1016/S0254-6299(15)31096-6
George, E. F., Hall, M. A., & De Klerk, G. J. (2008). Plant propagation by tissue culture (3rd ed., Vol. 1). https://doi.org/10.1007/978-1-4020-5005-3
Grootaarts, H., Schel, J. H. N., & Pierik, R. L. M. (1981). The origin of bulblets formed on excised twin scales of Nerine bowdenii W. Watts. Plant Cell, Tissue and Organ Culture, 1(1), 39–46. https://doi.org/10.1007/BF02318902
Hasegawa, A., Takigawa, Y., & Tokuzumi, A. (1995). Propagation of Eucharis grandiflora Planch. by tissue culture. Technical Bulletin of Faculty of Agriculture-Kagawa University (Japan), 47(1), 79–86.
Hong, J., & Lee, A. K. (2012). Micropropagation of Cyrtanthus ’Orange Gem’×C. eucallus hybrid. Scientia Horticulturae, 142, 174–179. https://doi.org/10.1016/j.scienta.2012.05.016
Meerow, A. W. (1989). Systematics of the Amazon Lilies, Eucharis and Caliphruria (Amaryllidaceae). Annals of the Missouri Botanical Garden, 76(1), 220. https://doi.org/10.2307/2399347
Meerow, A. W., Jost, L., & Oleas, N. (2015). Two new species of endemic ecuadorean Amaryllidaceae (Asparagales, Amaryllidaceae, Amarylloideae, Eucharideae). PhytoKeys, 48(1), 1–9. https://doi.org/10.3897/phytokeys.48.4399
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Ozel, C. A., Khawar, K. M., & Unal, F. (2015). Factors affecting efficient in vitro micropropagation of Muscari muscarimi Medikus using twin bulb scale. Saudi Journal of Biological Sciences, 22(2), 132–138. https://doi.org/10.1016/j.sjbs.2014.09.007
Paredes, K., Delaveau, C., Carrasco, P., Baeza, C., Mora, F., & Uribe, M. E. (2014). In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile. Ciencia e Investigacion Agraria, 41(2), 207–214. https://doi.org/10.4067/S0718-16202014000200007
Pierik, R. L. M., Sprenkels, P. A., & van Bragt, J. (1983). Rapid vegetative propagation of Eucharis grandiflora in vitro. Acta Horticulturae, (147), 179–186. https://doi.org/10.17660/actahortic.1983.147.25
Rodrigues-Martins, J. P., Pasqual, M., Martins, A. D., & Ribeira, S. F. (2015). Effects of salts and sucrose concentrations on in vitro propagation of Billbergia zebrina (Herbert) Lindley (Bromeliaceae). Australian Journal of Crop Science, 9(1), 85–91.
Santos, A., Fidalgo, F., Santos, I., & Salema, R. (2002). In vitro bulb formation of Narcissus asturiensis, a threatened species of the Amaryllidaceae. Journal of Horticultural Science and Biotechnology, 77(2), 149–152. https://doi.org/10.1080/14620316.2002.11511471
Siwach, P., Grover, K., & Gill, A. R. (2011). The influence of plant growth regulators, explant nature and sucrose concentration on in vitro callus growth of Thevetia peruviana Schum. Asian Journal of Biotechnology, 3(3), 280–292. https://doi.org/10.3923/ajbkr.2011.280.292
Smith, R. H., Burrows, J., & Kurten, K. (1999). Challenges associated with micropropagation of Zephyranthes and Hippesatrum sp. (Amaryllidaceae). In Vitro Cellular and Developmental Biology - Plant, 35(4), 281–282. https://doi.org/10.1007/s11627-999-0032-y
Sultana, J., Sultana, N., Siddique, M. N. A., Islam, A. K. M. A., Hossain, M. M., & Hossain, T. (2010). In vitro bulb production in Hippeastrum (Hippeastrum hybridum). Journal of Central European Agriculture, 11(4), 469–474. https://doi.org/https://doi.org/10.5513/JCEA01/11.4.867
Tipirtamaz, R. (2003). Rooting and acclimatization of in vitro micropropagated snowdrop (Galanthus ikariae Baker.) bulblets. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 16(2), 121–126.
Trigiano, R. N., & Gray, D. J. (2004). Plant development and biotechnology. https://doi.org/10.1201/9780203506561
Van Dijk, H., & Kurpershoek, M. (2003). La enciclopedia de las plantas bulbosas. Editorial LIBSA.
Vargas, T. E., Oropeza, M., & de García, E. (2006). Propagación in vitro de Hippeastrum sp. Agronomía Tropical, 56(4), 621–626.
Yusti-Muñoz, A. P., & Velandia-Perilla, J. H. (2013). First record of the amazon lily Eucharis sanderi (Amaryllidaceae) for Isla Gorgona, Colombia. Boletín Científico. Centro de Museos. Museo de Historia Natural, 17(1), 46–49.
Ziv, M., & Lilien-Kipnis, H. (2000). Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant Cell Reports, 19(9), 845–850. https://doi.org/10.1007/s002990000204
Publicado
2021-01-29
Cómo citar
Guerrero Valencia, F., Rodríguez de la O, J., Juárez Hernández, M., Ayala Arreola, J., & Ramírez González, G. (2021). Micropropagación del lirio amazónico (Eucharis grandiflora Planch. & Linden) mediante organogénesis directa. POLIBOTÁNICA, 1(51). Recuperado a partir de https://polibotanica.mx/index.php/polibotanica/article/view/912
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