Extractos vegetales para el control de Colletotrichum gloeosporioides in vitro y en periodo de floración y poscosecha del fruto de Carica papaya
Plant extracts for control of Colletotrichum gloeosporioides in vitro and blooming period and postharvest stage on Carica papaya
Palabras clave:
antracnosis, flores, frutos, hongos fitopatógenos, papaya, terpenoides.
Resumen
El objetivo fue evaluar la actividad de los extractos orgánicos de seis especies vegetales para el control de Colletotrichum gloeosporioides in vitro y en periodo de floración y poscosecha en el fruto de papaya (Carica papaya L.). Las variables evaluadas fueron efectividad biológica para la evaluación in vitro, efectividad en flores y severidad y efectividad en frutos poscosecha. El análisis fitoquímico de los extractos de las especies permitió identificar la presencia de flavonoides en S. rostratum y E. arvense; alcaloides en D. stramonium, M. parviflora y A. ochroleuca y terpenoides en P. icosandra. El extracto metanólico de P. icosandra mostró la mayor efectividad biológica in vitro contra el hongo C. gloeosporioides en comparación con las especies restantes. Por lo tanto, fue el único que se evalúo durante el periodo de floración y en poscosecha. La efectividad del extracto metanólico en la etapa de floración fue 60-70%, donde las flores mostraron necrosis y pudrición (síntomas típicos de la enfermedad) y 71.4% en el control de antracnosis en los frutos poscosecha.
Citas
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Ippolito, A., Schena, L., Pentimone, I., & Nigro, F. (2005).Control of postharvest rots of sweet cherries by pre- and postharvest applications of Aureobasidium pullulans in combination with calcium chloride or sodium bicarbonate. Postharvest Biology and Technology, 36(3), 245–252. https://doi.org/10.1016/j.postharvbio.2005.02.007
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Bautista, B. S., Barrera, N. L. L., Bravo, L. L., & Bermúdez, T. K. (2002). Antigunfal activity of leaf and stem extracts from various plant species on the incidence of Colletotrichum gloesporioides of papaya and mango fruit after storage. Revista Mexicana de Fitopatología, 20, 8-12.
Bogantes-Arias, A., & Mora-Newcomer, E. (2013). Incidencia y severidad de la antracnosis en líneas e híbridos de papaya (Carica papaya). Agronomía Mesoamericana, 24(2), 411-417.
Boumba, V. A., Mitselou, A., & Vougiouklakis, T. (2004). Fatal poisoning from ingestion of Datura stramonium seeds. Veterinary and Human Toxicology, 46(2), 81–2.
Brahmachari, G., Gorai, D., & Roy, R. (2013). Argemone mexicana: Chemical and pharmacological aspects. Brazilian Journal of Pharmacognosy, 23(3), 559-575. https://doi.org/10.1590/S0102-695X2013005000021
CABI (2016). Invasive Species Compendium. Wallingford, UK: CAB International. 10 -1-17. www.cabi.org/isc.
Chang, Y. C., Hsieh, P. W., Chang, F. R., Wu, R. R., Liaw C. C., Lee, K. H., & Wu, Y. C. (2003). Two new protopinesarge mexicaines A and B and the anti-HIV alkaloid 6-acetonyl dihydrochelerythrine from formasan Argemone mexicana. Planta Medica, 69, 148-152. https://doi.org/10.1055/s-2003-37710
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Doyle, J. J., & Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13-15.
Farhan, H., Rammal, H., Hijazi, A., Hamad, H., Daher, A., Reda, M., & Badran, B. (2012). In vitro antioxidant activity of ethanolic and aqueous extracts from crude Malva parviflora L. grown in Lebanon. Asian Journal of Pharmaceutical and Clinical Research, 5, 234-238.
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Guerrero, R. E., Solís, G, S., Hernández C, F. D., Flores, O. A., Sandoval L. V., & Jasso, C. D. (2007). Actividad biológica in vitro de extractos de Flourensia cernua DC en patógenos de postcosecha: Alternaria alternata (Fr.: Fr.) Keissl., Colletotrichum gloeosporioides (Penz.) Penz. y Sacc. y Penicilliu< digitatum (Pers.: Fr.) Sacc. Revista Mexicana de Fitopatología, 25(1), 48-53.
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Haiyan, L., Chen, Q., Yanli, Z., & Zhiwei, Z. (2005). Screening for endophytic fungi with antitumour and antifungal activities from Chinese medicinal plants. World Journal of Microbiology and Biotechnology, 21, 1515-1519. https://doi.org/10.1007/s11274-005-7381-4
Hernández, V. M. M., Borges, A. R., Rodriguez, V. R. I., Torres, A. J. F. J., Méndez, G. M., & Caceres, F. M. (2011). Ovicidal and larvicidal activity of the crude extracts from Phytolacca icosandra against Haemonchus contortus. Veterinary Parasitology, 179(1), 100-106. https://doi.org/10.1016/j.vetpar.2011.02.019
Hernández, M., Murace, M., Ringuelet, J., Petri, I., Gallo, D., & Arambarri, A. (2013). Effect of aqueous and alcohol Extracts of Phytolacca tetramera (Phytolaccaceae) leaves on Colletotrichum gloeosporioides (Ascomycota). Boletín de la Sociedad Argentina de Botánica, 48 (2), 201-209. https://doi.org/10.31055/1851.2372.v48.n2.6205
Ippolito, A., Schena, L., Pentimone, I., & Nigro, F. (2005).Control of postharvest rots of sweet cherries by pre- and postharvest applications of Aureobasidium pullulans in combination with calcium chloride or sodium bicarbonate. Postharvest Biology and Technology, 36(3), 245–252. https://doi.org/10.1016/j.postharvbio.2005.02.007
Leslie, J. F., & Summerell, B. A. (2008). The Fusarium Laboratory Manual. John Wiley & Sons. Blackwell. Manhattan. 387 p.
Mshvildadze, V., Favel, A., Delmas, F., Elias, R., Faure, R., Decanosidze, G., Kemertelidze, E., & Balansard, G. (2000). Antifungal and antiprotozoal activities of saponins from Hedera colchica. Pharmazie, 55, 325–326.
Mimica, D. N., Simin, N. C. J., Jovin, E., Orcic, D., & Bozin, B. (2008). Phenolic compounds in field horsetail (Equisetum arvense L.) as natural antioxidants. Crop and Pasture Science, 13, 1455–1464. https://doi.org/10.3390/molecules13071455
Omar, T., Noman, L., Mohamed1, B., Altuntas, F. O., & Demirtas, I. (2018). Phytochemical constituents and antioxidante effect of Solanum rostratum species from Argelia. Asian J Pharm Clin Res, 11 6, 219-223
Ong, M. K., & Ali, A. (2015). Antifungal action of ozone against Colletotrichum gloeosporioides and control of papaya anthracnose. Postharvest Biology and Technology, 100, 113–119. https://doi.org/10.1016/j.postharvbio.2014.09.023
Regnault, R. C., Philogene, B. J., Terrón, P. U., & Vincent, C. (2004). Biopesticidas de Origen Vegetal. Madrid España. Mundiprensa.
SAS, Institute. (2003). SAS/STAT. User´s Guide. Release 9.0 ed. SAS Institute Inc., Cary, North Carolina. USA.
Segal, R., & Schlösser, E. (1975). Role of glycosidases in the membranlytic, antifungal action of Saponins. Archives of Microbiology, 104, 147-150.
Sharma, M., & Kulshrestha, S. (2015). Colletotrichum gloeosporioides: An anthracnose causing pathogen of fruits and vegetables. Biosciences Biotechnology Research Asia, 12(2), 1233–1246. Available in: https://doi.org/10.13005/bbra/1776
Singh, S., Singh, A., Keshariwala, M., Singh, T. D., Singh, V. P., Pandey, V. B., & Singh, U. P. (2010). The mixture of tertiary and quaternary alkaloids isolated from Argemone ochroleuca inhibits spore germination of some fungi. Archives of Phytopathology and Plant Protection, 43, 1249-1253. https://doi.org/10.1080/03235400802404627
Solano, V., & Arauz, L. F. (1995). Combate de antracnosis en frutos de papaya mediante aplicaciones de fungicidas en el campo en la zona atlantica de costa rica. Agronomia Costarricense, 19(2), 25–30.
Tadeg, H., Mohammed, E., Asres, K., & Gebre, M. T. (2005). Antimicrobial activities of some selected traditional Ethiopian medicinal plants used in the treatment of skin disorders. Journal of Ethnopharmacology, 100, 168-175. https://doi.org/10.1016/j.jep.2005.02.031
Talhinhas, P., Sreenivasaprasad, S., Neves, M. J., & Oliveira, H. (2002).Genetic and morphological characterization of Colletotrichum acutatum causing anthracnose of Lupins. Phytopathology, 92(9), 986–996. https://doi.org/10.1094/PHYTO.2002.92.9.986.
Tapia-Tussell, R., Quijano-Ramayo, A., Cortes-Velázquez, A., Lappe, P., Larqué-Saavedra, A., & Pérez-Brito, D. (2008). PCR-based detection and characterization of the fungal pathogens Colletotrichum gloeosporioides and Colletotrichum capsici causing anthracnose in papaya (Carica papaya L.) in the Yucatán peninsula. Molecular Biotechnology, 40(3), 293- 298. https://doi.org/10.1007/s12033-008-9093-0.
Tatagiba, J., Liberato, J., Zambolim, L., Ventura, J., & Costa, H. (2002). Control y condiciones climáticas favorables a antracnosis de papaya. Fitopatología Brasileira, 27(2), 186-192.
Tavares, W. de S., Cruz, I., Petacci, F., de Assis Júnior, S. L., de Sousa Freitas, S., Zanuncio, J. C., & Serrão, J. E. (2009). Potential use of Asteraceae extracts to control Spodoptera frugiperda (Lepidoptera: Noctuidae) and selectivity to their parasitoids Trichogrammapretiosum (Hymenoptera: Trichogram matidae) and Telenomus remus (Hymenoptera: Scelionidae). Industrial Crops and Products, 30(3), 384–388. https://doi.org/10.1016/j.indcrop.2009.07.007
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Publicado
2021-01-30
Cómo citar
García.-Mateos, M. del, Acosta Ramos, M., Rodriguez-Pérez, E., Hernandez-Ramos, L., & Vazquez Sánchez, J. (2021). Extractos vegetales para el control de Colletotrichum gloeosporioides in vitro y en periodo de floración y poscosecha del fruto de Carica papaya. POLIBOTÁNICA, 1(51). Recuperado a partir de https://polibotanica.mx/index.php/polibotanica/article/view/907
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